猪卵巢发育的组织学变化及卵泡闭锁规律研究

doi:10.11843/j.issn.0366-6964.2017.10.009

摘要/Abstract

摘要：

旨在对不同日龄猪卵巢组织结构以及卵巢发育过程中卵泡闭锁规律进行研究。取3、40、50、60、72、86、95及165日龄猪卵巢各3例，采用常规石蜡切片、HE染色和TUNEL技术检测卵巢组织结构及卵泡闭锁规律，结果表明，猪卵巢发育从组织学上可分为卵原细胞增殖期、卵泡缓慢生长期及卵泡快速生长期3个阶段。卵原细胞增殖期主要以卵原细胞的增殖分裂为特点；在卵泡缓慢生长期，原始卵泡的数量随日龄增加逐渐减少，初级卵泡数量及体积则明显增加；至卵泡快速生长期，生长卵泡的数量及体积继续增大，其数量在86日龄达到最大值；72日龄卵巢中出现三级卵泡，至95日龄卵巢中出现近成熟卵泡。在各时期的卵巢组织，均可观察到卵原细胞及卵泡的闭锁现象，以原始卵泡的退化最为显著。TUNEL检测表明，在卵原细胞增殖期，可见大量原始卵泡及初级卵泡的闭锁，其闭锁主要源自卵泡卵母细胞的凋亡。在卵泡缓慢生长期，各级生长卵泡均可出现闭锁，初级卵泡的闭锁主要由卵母细胞的凋亡引起，也有部分是同时伴有卵母细胞和卵泡细胞凋亡的；次级卵泡的闭锁则主要由于颗粒层细胞的凋亡所致。在卵泡快速生长期，随着卵泡的快速生长，各级卵泡的闭锁也变得更加明显，次级卵泡及三级卵泡的闭锁主要是由颗粒细胞凋亡引起。

Abstract:

The study aimed to analyze the histological structures at different ages of postnatal porcine ovary and rules of follicular atresia during ovarian development. Three pig ovary samples from 3,40,50,60,72,86,95 and 165 postnatal days were collected, respectively, and these samples were examined by using common paraffin sectioning method,HE staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technology. The results showed that the histological development process of pig ovary was divided into 3 periods:proliferation of oogonia, slow growth of follicles and rapid growth of follicles. Proliferation of oogonia period was characterized by the proliferation of oogonia. In slow growth of follicles period, the number of primordial follicles gradually declined with ages, while the number and the volume of primary follicles evidently increased. In rapid growth of follicles period, both the number and the volume of growing follicles continued to increase, and the number reached its maximum at 86 postnatal days. Tertiary follicles were first observed in ovary at 72 postnatal days, and nearly mature follicles appeared at 95 postnatal days, respectively. In all 3 periods, the phenomenon of follicular atresia could be observed both in oogonia and follicles. Eespecially, the atresia in primordial follicles were the most significant. TUNEL staining showed that, in proliferation of oogonia period, the atresia of a great number of primordial follicles and primary follicles could be observed, which were mainly caused by apoptosis on follicular oocytes. In slow growth of follicles period, phenomenon of atresia occured in all kinds of growing follicles. Primary follicular atresia was mainly caused by apoptosis on oocyte, some of them were caused by the apoptosis both on oocytes and follicular cells. Secondary follicular atresia was mainly due to apoptosis of layers of the granular cells. In rapid growth of follicles period, with the rapid growth of follicles, the phenomenon of atresia occured in all kinds of follicles became more evident. The atresia in tertiary follicles and secondary follicles were mainly caused by the apoptosis of granulosa cells.

中图分类号:

S852.16

梁学超, 蒋明, 罗玉茹, 张为民, 卿素珠. 猪卵巢发育的组织学变化及卵泡闭锁规律研究[J]. 畜牧兽医学报, 2017, 48(10): 1863-1870.

LIANG Xue-chao, JIANG Ming, LUO Yu-ru, ZHANG Wei-min, QING Su-zhu. Study on Histology and Patterns of Follicular Atresia during Ovarian Development in Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1863-1870.

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https://www.xmsyxb.com/CN/Y2017/V48/I10/1863

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